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J. Biochem, 1992, Vol. 112, No. 6 733-736
© 1992 Japanese Biochemical Society

research-article

Major ATPases on Clofibrate-Induced Rat Liver Peroxisomes Are Not Associated with 70 kDa Peroxisomal Membrane Protein (PMP70)1

Sakae Shimizu*, Tsuneo Imanaka**, Tatsuya Takano** and Shoji Ohkuma*,1

*Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University 13-1 Takara-machi, Kanazawa, Ishikawa 920
**Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa 199-01

2To whom correspondence should be addressed

We previously reported that novel Mg2+-ATPases were induced in rat liver peroxisomes by clofibrate administration and that these activities consisted of at least two types of enzymes, N-ethylmaleimide (NEM)-sensitive and -resistant. Here we present evidence that neither of these major peroxisomal ATPases is associated with the 70-kDa peroxisomal membrane protein (PMP70), because: (i) proteinase K treatment of peroxisomes resulted in inactivation of only NEM-sensitive ATPase, whereas disappeared PMP70 completely; (ii) NEM-sensitive ATPase activity was barely immunoprecipitated with anti-PMP70 IgG; (iii) the solubilized ATPases behaved differently from PMP70 on native PAGE; and finally (iv), the major peroxisomal ATPases were separated from PMP70 on gel filtration chromatography.

1This work was supported in part by Research Funds from Sankyo Foundation of Life Science, Terumo Life Science Foundation, and the Hokuriku Industrial Advancement Center, the Project Research Fund from Kanazawa University, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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