J. Biochem, 1992, Vol. 112, No. 6 743-749
© 1992 Japanese Biochemical Society
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Chain Length Distribution of the Products Formed in Solanesyl Diphosphate Synthase Reaction1
Institute for Chemical Reaction Science, Tohoku University 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980
Factors that affect the termination of isoprenoid chain elongation catalyzed by prenyltransferase were investigated. The chain-length distribution of reaction products of solanesyl diphosphate synthase [EC 2.5.1.11 [EC] ] homogeneously purified from Micrococcus luteus changed dramatically according to the concentration of the complex formed between isopentenyl diphosphate and Mg2+ (IPP-Mg) in the reaction mixture. However, the concentration of the complex between farnesyl diphosphate and Mg2+ (FPP-Mg), the priming substrate for this synthase, did not affect the product distribution, provided that the concentration of IPP-Mg was maintained at a certain level. Thus, the level of IPP-Mg is decisive in affecting the chain length distribution of the products of the prenyltransferase reaction, and the Mg2+-dependent variability of product specificity so far observed can now be understood in terms of the effect of IPP-Mg concentration.
1 This research was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan and the Special Coordination Funds of the Science and Technology Agency of Japan.
2 Present address: Department of Biochemistry and Engineering, Faculty of Engineering, Tohoku University, Aramaki, Aoba-ku, Sendai, Miyagi 980
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