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J. Biochem, 1992, Vol. 112, No. 6 856-863
© 1992 Japanese Biochemical Society

research-article

Molecular Cloning and Partial Characterization of a Novel Collagen Chain, {alpha}l(XVI), Consisting of Repetitive Collagenous Domains and Cysteine-Containing Non-Collagenous Segments

Noriko Yamaguchi*, Shioko Kimura**, O. Wesley McBride***, Hisae Hori*, Yoshihiko Yamada****, Tetsuko Kanamori*, Hiroaki Yamakoshi* and Yutaka Nagai*,1

*Department of Tissue Physiology, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo 101
**Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, MD 20892, U.S.A.
***Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892, U.S.A.
****Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Bethesda, MD 20892, U.S.A.

2To whom correspondence should b addressed

In an effort to identify new members of the collagen family, we screened a human placenta cDNA library with a collagenous probe. A novel 3.7 kb cDNA was identified encoding an open reading frame of 1,186 amino acids and containing a termination codon. The predicted polypeptide consists of 9 repetitive collagenous (stretches of Gly-X-Y) and several non-collagenoue segments. Two cysteinyl residues separated by two amino acid residues (Cys-X-X-Cys) are regularly located in the N-terminal region of each non-collagenous segment. The deduced amino acid sequence described above is distinct from those of known types of collagen. Therefore, this novel collagen chain is designated {alpha}1(XVT). Northern blot analysis revealed an {alpha}1(XVI) mRN A of 5.2 kb, indicating that the overlapping cDNA clones isolated in this study covered nearly three-fourths of the mRNA. As a tool for further study on the expression of type XVI collagen, we prepared an antibody against the nonadecapep-tide CFLSLERPRAEEARGDNSE, derived from the putative translation product of the cDNA. In immunoblot analysis, the antibody recognized a 160 kDa protein, which was bacterial collagenase-sensitive. Immunohistochemical stainings of human placental tissues with anti-peptide antibody revealed a positive reaction with amnion, the membranous tissue lining the amniotic cavity. The gene of {alpha}1(XVI) chain, COL16A1, is mapped on the short arm of human chromosome 1 (1p13-p34).


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