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J. Biochem, 1993, Vol. 113, No. 3 299-303
© 1993 Japanese Biochemical Society

research-article

A Membrane-Bound Metallo-Endopeptidase from Rat Kidney: Its Immunological Characterization1

Tom Yamaguchi*,2, Hiroshi Kido**, Riko Kitazawa*, Sohei Kitazawa***, Masaaki Fukase* and Nobuhiko Katunuma**,3

*Third Division, Department of Medicine, Kobe University School of Medicine Chuo-ku, Kobe 650
**Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima Tokushima, Tokushima 770
***Second Department of Pathology, Kobe University School of Medicine Chuo-ku, Kobe 650

2To whom correspondence should be addressed.

The structure and location of a membrane-bound metallo-endopeptidase, previously purified from rat kidney [Yamaguchi et al. (1991) Eur. J. Biochem. 200, 563–571], were examined by immunochemical and immunohistochemical methods with a rabbit polyclonal antibody against the purified enzyme. On treatment with endoglycosidase F, the subunit of the purified enzyme (molecular mass = 88 kDa) was converted to a smaller form (78.5 kDa), indicating that the enzyme contained at least 11% iV-linked carbohydrate. Treatment of kidney membranes with papain resulted in release of the enzyme, as shown by Western blotting analysis of the solubilized fraction. Immunoassays of rat tissues showed that only the kidney, and small and large intestine expressed significant amounts of the antigen. Moreover, immunohistochemical studies showed that the antigen was confined to the luminal surfaces of the proximal renal tubules and the intestinal villi. Thus, like another kidney membrane metallo-endopeptidase, meprin [Kounnas et al. (1991) J. Biol. Chem. 266, 17350–17357], the purified enzyme is shown to be a glycoprotein that is probably anchored in the plasma membrane, and located in the luminal surface of microvillar membranes of the kidney and intestine. These results indicate that our enzyme and meprin have clear structural and topological similarities.

1This study was supported by research grants from the Ministry of Education, Science and Culture, and a grant from the Smoking Research Foundation of Japan.

3Present address: Health Science Institute, Bunri University, Tokushima 770.


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