Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Xu, G.
Right arrow Articles by Suzuki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, G.
Right arrow Articles by Suzuki, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1993, Vol. 113, No. 3 304-307
© 1993 Japanese Biochemical Society

research-article

Drift of the Sialyl-Linkage Specific Recognition of the Sialidase of Influenza B Virus Isolates1

Guiyun Xu*, Takashi Suzuki*, Gorou Hanagata**, Eiki Deya**, Makoto Kiso***, Akira Hasegawa*** and Yasuo Suzuki***

*Department of Biochemistry, University of Shizuoka School of Pharmaceutical Science Shizuoka, Shizuoka 422
**Technical Research Institute, Snow Brand Milk Products Co., Ltd. Kawagoe, Saitama 350
***Department of Applied Bio-organic Chemistry, Gifu University Gifu, Gifu 501-11

Sialyl-linkage specificity of the sialidase of influenza B viruses isolated in different years from 1940 through 1990 (B/Lee/40, B/Setagaya/3/56, B/Tokyo/7/66, B/Kagoshima/1/68, B/ Gifu/2/73, B/Kanagawa/3/76, B/Ibaraki/2/85, B/Yamagata/16/88, and B/Bangkok/163/ 90) was studied with. W-acetylneuraminyl (a2-3)- and (a2-6)-lactoses, GM3 gangliosides containing the same sialyl-oligosaccharide sequences as sialyllactose, and also with type I and type II lacto-series gangliosides carrying Neu5Acc2-3Gal and Neu5Aca2-6Gal linkages as substrates. From an examination of up to nine strains, the sialidases of all viruses preferentially hydrolyze substrates with Neu5Ac ar2-3Gal linkage rather than the Neu5A{alpha}2–3Gal linkage. It was found that the sialidase activity toward Neu5Aca2-6Gal linkage relative to Neu5Aca2-3Gal linkage is increased in later strains, whether sialyllactose or ganglioside is used as the substrate. These results suggested that the sialidase of influenza B virus isolates has shown a drift in linkage specificity which correlates with the year of isolation.

1This work was supported in part by a Grant in-Aid for Scientific Research in Priority Areas (01308028) (to Y.S. and A.H.) from the Ministry of Education, Science and Culture of Japan and Monbusho International Scientific Research Program: Joint Research (03044121) (toY.S.).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
T. Suzuki, A. Portner, R. A. Scroggs, M. Uchikawa, N. Koyama, K. Matsuo, Y. Suzuki, and T. Takimoto
Receptor Specificities of Human Respiroviruses
J. Virol., May 15, 2001; 75(10): 4604 - 4613.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.