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J. Biochem, 1993, Vol. 113, No. 3 327-333
© 1993 Japanese Biochemical Society

research-article

Effects of Ryanodine on Permeability of Choline and Glucose through Calcium Channels inSarcoplasmic Reticulum Vesicles1

Michiki Kasai and Takashi Kawasaki

Department of Biophysical Engineering, Faculity of Engineering Science, Osaka University Osaka 560

The effect of ryanodine on the permeation of glucose, choline, and Ca2+ through the Ca2+ channel in sarcoplasmic reticulum vesicles was studied by means of the light scattering and/or the tracer method. Ryanodine had the same effect on the permeabilities of choline and Ca2+; low concentrations of ryanodine (10 ptM) locked the channel in the open state, and high concentrations (100 /xM) opened the channel transiently, which closed finally. Glucose became permeable on the ryanodine treatment even in the absence of KC1, although it was not permeable before the treatment when the channel was open. Submolar concentrations of KC1 enhanced the glucose and choline permeability of the ryanodine-treated channel as well as the untreated one. Activators such as ATP and caffeine did not enhance the permeability of the treated channel in the open locked state, butinhibitors such as Mg2"1" and ruthenium red closed the channel, although much higher concentrations were required than for the untreated channel. The maximal rates of choline and glucose permeation of the ryanodine-treated channel were lower than those of the untreated one attained in the presence of ATP and/or caffeine. This result is consistent with the fact that the single channel conductance after the ryanodine treatment decreased to 40-50% of the maximal conductance of the untreated one. This result suggests that the conformation of the channel opened by ryanodine was different from that with ATP or caffeine.

1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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