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J. Biochem, 1993, Vol. 114, No. 2 279-283
© 1993 Japanese Biochemical Society

other

Role of Myosin in the Stimulatory Effect of Caldesmon on the Interaction between Actin, Myosin, and ATP1

Yuan Lin2, Ryoki Ishikawa and Kazuhiro Kohama3

Department of Pharmacology, Gunma University School of Medicine Maebashi, Gunma 371
2On leave of absence from the Department of Pharmacology, Dalian Medical College Dalian, P.R. of China.

3To whom correspondence should be addressed.

We have previously shown that caldesmon at low concentrations stimulates the interaction between actin, myosin, and ATP, while at high concentrations it inhibits the interaction [Ishikawa, R., Okagaki, T., Higashi-Fujime, S., & Kohama, K. (1991) J. Biol. Chem. 266, 21784–21790], When the effect of caldesmon at low concentrations was monitored by measuring myosin ATPase activity in the absence of actin, the effect was slightly but significantly stimulatory; and at higher concentrations no inhibitory effect was observed. Therefore, we related the stimulatory effect with the myosin-binding property of caldesmon. In the presence of actin, a low concentration of caldesmon was not enough to evince the stimulatory effect: myosin concentration must also be low. This is because the stimulatory effect was obscured when myosin concentration was elevated. Ca2+-calmodulin abolished the stimulatory effect of caldesmon. However, the concentration of calmodulin required to abolish the stimulation was higher than that required to abolish the inhibition.

1 This study was supported by grants from Suzuken Memorial Foundation, Bayer Yakuhin Ltd., Uehara Memorial Foundation, and the Japan-China Medical Association and by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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