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J. Biochem, 1993, Vol. 114, No. 2 292-296
© 1993 Japanese Biochemical Society

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Two Mouse Hypervariable Minisatellites: Chromosomal Location and Simultaneous Mutation1

Satoshi Suzuki*, Eohnosuke Mitani*, Katsuhiro Kuwabara*, Yoshiaki Takahashi*, Ohtsura Niwa** and Ryo Kominami*

*First Department of Biochemistry, Niigata University School of Medicine 1-757 Asahimachi-doori, Niigata, Niigata 951
**Department of Pathology, Research Institute for Nuclear Medicine and Biology, Hiroshima University 1-2-3 Kasumi, Minami-ku, Hiroshima 734

Pc-1 and Pc-2 hypervariable minisatellites of the mouse show a structural similarity and hence have been compared in terms of chromosomal location and mutability. Polymorphisms of the Pc-2 locus detected by analyses of Southern blotting and polymerase chain reaction were used for typing 61 backcross mice that were obtained by mating (C67BL/6x MSM)F1 females to MSM males. Segregation patterns indicated that the Pc-2 locus maps to the 60 cM region of chromosome 6, while that of Pc-1 maps to chromosome 4. Simultaneous mutation of the two loci was examined in 101 F1 mice. Pc-1 and Pc-2 loci showed mutation rates of 14.4 and 5.0% per gamete, respectively. Of the nine mice with a Pc-2 mutation, four carried a Pc-1 mutation at the same maternally- or paternally-derived allele. The rate of comutation was 44% (4/9), higher than the 12.2% (24/197) of Pc-1 mutation alone. This suggests that the germline mutation of the two hypervariable minisatellites may result from a common mechanism.

1This work was supported by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan.


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