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J. Biochem, 1994, Vol. 115, No. 2 175-178
© 1994 Japanese Biochemical Society

review-article

Protozyme: Emerging Evidence in Nature1

Yasuhiro Anraku* and Ryogo Hirata**

*Department of Plant Sciences, Graduate School of Science, The University of Tokyo Bunkyo-ku, Tokyo 113
**The Institute of Chemical and Physical Research (RIKEN) Wako, Saitama 351-01

The VMA1 locus in Saccharomyces cerevisiae contains a nested genetic element, the VDE gene, and expresses two functional proteins. A single VMA1 translational product seems to catalyze a self protein splicing in which an internal domain is excised out to produce a site-specific DNA endonuclease and the N- and C-domains are ligated by a transpeptidation reaction to yield a catalytic subunit of the vacuolar ATPase. Accumulating evidence in the past few years suggests that the VMA1 locus encodes a protozyme (for protos en zyme) which has dual roles in life as a protean catalyst for self protein splicing and for self gene homing. Four protozymes that share a common mechanism in protein splicing have been found in four organisms covering three major phylogenic trees.

1Work in our laboratory is supported by a Grant-in-Aid for Scientific Research on Priority Areas of Cellular Energy from the Ministry of Education, Science and Culture of Japan.


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M. Kawasaki, S. Nogami, Y. Satow, Y. Ohya, and Y. Anraku
Identification of Three Core Regions Essential for Protein Splicing of the Yeast Vma1 Protozyme. A RANDOM MUTAGENESIS STUDY OF THE ENTIRE VMA1-DERIVED ENDONUCLEASE SEQUENCE
J. Biol. Chem., June 20, 1997; 272(25): 15668 - 15674.
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