Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Miyatake, N.
Right arrow Articles by Tsugita, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyatake, N.
Right arrow Articles by Tsugita, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1994, Vol. 115, No. 2 208-212
© 1994 Japanese Biochemical Society

research-article

Specific Chemical Cleavage of Asparaginyl and Glycyl-Glycine Bonds in Peptides and Proteins by Anhydrous Hydrazine Vapor1

Norifumi Miyatake*,**, Kazuo Satake*, Masaharu Kamo* and Akira Tsugita*,2

*Research Institute for Biosciences, Science University of Tokyo 2669 Yamazaki, Noda, Chiba 278
**Department of Applied Biosciences, Science University of Tokyo 2669 Yamazaki, Noda, Chiba 278

2To whom correspondence should be addressed.

Hydrazinolysis of peptide or protein has been used for C-terminal amino acid determination by Akabori et al. (1952). In this study, proteins were reacted with anhydrous hydrazine vapor at 20°C for 16 h. Asparaginyl linkages were cleaved. Asparagine and glutamine were converted to their hydrazides, ß-hydrazidyl aspartic acid and {gamma}-hydrazidyl glutamic acid, respectively, even under milder conditions. The former hydrazide cyclizes to a 6-membered ring, asparaginyl bond at the carboxyl side. Other cleavages, including the glycyl-glycine bond, were also observed.

1This work was supported by a Grant-m-Aid for Specially Promoted Research form the Ministry of Education, Science and Culture of Japan This paper is dedicated to the late Professor Shiro Akabon, Osaka University.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.