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J. Biochem, 1994, Vol. 115, No. 2 286-292
© 1994 Japanese Biochemical Society

research-article

Transcription of the faoAB Operon Which Encodes the HDT Multienzyme Complex Involved in Fatty Acid ß-Oxidation in Pseudomonas fragi B-07711

Shigeharu Sato2, Yoshihiro Ozeki and Akihiko Kawaguchi3

Department of Biology, College of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153

3To whom reprints requests should be addressed.

Transcription of the two clustered genes, faoA and faoB, for the multienzyme complex for fatty acid ß-oxidation (HDT) from the Gram-negative bacterium, Pseudomonas fragi, was investigated. Northern blot hybridization and primer extension analysis indicated that these genes comprised an operon, giving a 3.6 kb mRNA. Transcription of the 3.6 kb faoAB mRNA was induced by palmitic acid. Using the lacZ gene as a reporter gene for a promoter activity search, the essential region for induction of the faoAB gene transcription by palmitic acid was mapped at the 331 by region just upstream of the transcription initiation site. Deletion of the 136 by Sall-Nhel fragment just downstream of the transcription initiation site caused the constitutive expression of the faoA-lacZ fusion protein in P.fragi, suggesting that there is a regulatory element interacting with the transcription repressor.

1This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

2Present address: The Zoological Institute, Faculty of Science, The University of Tokyo.


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