J. Biochem, 1994, Vol. 115, No. 2 298-303
© 1994 Japanese Biochemical Society
research-article |
Novel Isoforms of Human Cyclic AMP-Responsive Element Modulator (hCREM) mRNA1
Department of Cellular and Molecular Biology, Institute of Medical Science, The University of Tokyo, 4–6–1 Shirokanedai Minato-ku, Tokyo 108
The cyclic AMP-response element (CRE), a transcriptional enhancer, is regulated by CREB (CRE-binding protein) which is the leucine zipper protein phosphorylated by protein kinase A in response to cAMP signal. The highly homologous protein CREM (CRE-modulator) is thought to modulate CREB-stimulated transcription, and is also involved in transcriptional control during spermatogenesis. In this paper, we report two types of cDNAs of human CREM (hCREM), type 1 and type 2; type 1 is a group of human counterparts of the mouse CREM
and type 2 is a novel form having a distinct 5' exon which is unrelated to any species of the CREB and CREM isoforms so far described. This unique 5' region of type 2 hCREM may suggest its independent expression from type 1 CREM. The specific 5' region of type 2 hCREM consisted of 88 bp, containing an initiation codon for translation, but no possible phosphorylation site, suggesting different roles from type 1 CREM. Both type 1 and 2 hCREMs are expressed in lymphoid and non-lymphoid cell lines. Their excess expression by transfection induced suppression of cAMP-mediated activation of transcription, suggesting their negative regulation of CRE-mediated transcription.
1This work was supported in part by a grant for Special Project Research, Cancer Bioscience, from the Ministry of Education, Culture and Science of Japan. The sequences described here been submitted to DDBJ under accession numbers D14825 [GenBank] and D14826 [GenBank] .
2Present address: Department of Microbiology, Keio University, School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo 160
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Pohnke, T. Schneider-Merck, J. Fahnenstich, R. Kempf, M. Christian, K. Milde-Langosch, J. J. Brosens, and B. Gellersen Wild-Type p53 Protein Is Up-Regulated upon Cyclic Adenosine Monophosphate-Induced Differentiation of Human Endometrial Stromal Cells J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5233 - 5244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Gellersen, R. Kempf, R. Sandhowe, G. F. Weinbauer, and R. Behr Novel leader exons of the cyclic adenosine 3',5'-monophosphate response element modulator (CREM) gene, transcribed from promoters P3 and P4, are highly testis-specific in primates Mol. Hum. Reprod., November 1, 2002; 8(11): 965 - 976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Behr, N. Hunt, R. Ivell, J. Wessels, and G.F. Weinbauer Cloning and Expression Analysis of Testis-Specific Cyclic 3',5'-Adenosine Monophosphate-Responsive Element Modulator Activators in the Nonhuman Primate (Macaca fascicularis): Comparison with Other Primate and Rodent Species Biol Reprod, May 1, 2000; 62(5): 1344 - 1351. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Bockmann, T. M. Bockers, C. Winter, W. Wittkowski, H. Winterhoff, Th. Deufel, and M. R. Kreutz Thyrotropin Expression in Hypophyseal Pars Tuberalis-Specific Cells is 3,5,3'-Triiodothyronine, Thyrotropin-Releasing Hormone, and Pit-1 Independent Endocrinology, March 1, 1997; 138(3): 1019 - 1028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Gellersen, R. Kempf, and R. Telgmann Human Endometrial Stromal Cells Express Novel Isoforms of the Transcriptional Modulator CREM and Up-Regulate ICER in the Course of Decidualization Mol. Endocrinol., January 1, 1997; 11(1): 97 - 113. [Abstract] [Full Text]
|
|