Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Ahmad, F.
Right arrow Articles by Haque, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ahmad, F.
Right arrow Articles by Haque, S. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1994, Vol. 115, No. 2 322-327
© 1994 Japanese Biochemical Society

research-article

A New Method for Testing the Functional Dependence of Unfolding Free Energy Changes on Denaturant Concentration1

Faizan Ahmad2, Superna Taneja, Sushma Yadav and Syed Ehtaishamul Haque

Department of Chemistry, Jamia Makes Islamia Jamia Nagar, New Delhi 100 025, India

2To whom correspondence should be addressed.

Denaturations of ribonuclease A, lysozyme, and cytochrome c by guanidine hydrochloride (GdnHCI), urea, and GdnHCl-urea mixture were studied at constant temperature and pH to assess the functional dependence of denaturational free energy change ({Delta}GD) on denaturant concentration over an extended GdnHCl concentration range. Conventional analysis of GdnHCl-induced transition curve exhibits a linear plot of {Delta}GD, versus [GdnHCl] in the transition zone. To extend {Delta}GDmeasurements beyond this narrow concentration range, GdnHCl-induced unfolding was measured in the presence of different concentrations of urea. {Delta}GD values from these measurements were corrected for the effect of urea on the free energy change using the appropriate relation. The corrected {Delta}GD data were mapped onto the {Delta}GD versus [GdnHCl] plot. For each protein, the dependence of free energy change on denaturation was found to be linear over the full GdnHCl concentration.

1This work was supported by a Grant from the Council of Scientific and Industrial Research, India, to Dr. Faizan Ahmad.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.