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J. Biochem, 1995, Vol. 118, No. 1 56-66
© 1995 Japanese Biochemical Society

other

Biochemical Characterization of Liver Microsomal, Golgi, Lysosomal, and Serum ß-Glucuronidases in Dibuty1 Phosphate-Treated Rats1

Yukio Nishimura2, Keitaro Kato and Masaru Himeno

Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku, Fukuoka 812-82

2 To whom correspondence should be addressed.

Organophosphate compounds are known to cause the selective release of rat liver microsomal ß-glucuronidase into plasma. To investigate the alterations of molecular forms and oligosaccharide moieties of liver ß-glucuronidase in organophosphate compound-administered rats, ß-glucuronidase was isolated from microsomal, Golgi, lysosomal, and serum fractions. In SDS-polyacry lamide gel electrophoresis, a single polypeptide band was observed on gels in Golgi and serum ß-glucuronidases. This result indicated that Golgi and serum ß-glucuronidases of treated rats did not undergo post-translational proteolytic processing, in contrast to those in control rat livers. Biochemical characterization of the isolated ß-glucuronidases by employing lectin affinity chromatography revealed that interaction of serum and Golgi enzymes with Ricinus communis agglutinin- and wheat germ agglutinin-Sepharose was fairly strong, and that microsomal and lysosomal enzymes were poorly retained on those columns. These results suggested that the serum and Golgi ß-glucuronidases are sialoglycoproteins. A clearance study also showed that infused serum ß-glucuronidase was slowly cleared from plasma with a half-life of about 60 min, but the asialo-serum enzyme was rapidly cleared with a half-life of about 5 min. These results imply that microsomal ß-glucuronidase undergoes extensive modification of the oligosaccharide moieties by terminal glycosyltransferases at the trans Golgi when it is destined for secretion into serum in response to treatment with an organophosphate compound.

1 This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.


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