J. Biochem, 1995, Vol. 118, No. 3 494-499
© 1995 Japanese Biochemical Society
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Nucleotide Sequence of a Principal Sigma Factor Gene(hrdB) of Streptomyces griseus1
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University 1 4-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739
2 To whom correspondence should be addressed. Phone: + 81-824-24-7768, Fax: +81-824-22-7191, e-mail: hshinka{at}ue.ipc.hiroshima-u.ac.jp
The hrdB homologue was isolated from a streptomycin-producing Streptomyces griseus 2247 strain, which is independent of A-factor. The nucleotide sequence of the cloned DNA fragment revealed the presence of an open reading frame (ORF) of 1,542 bp, which predicted a primary product of 514 amino acids and Mv 56,100. The N-terminal sequence of the purified HrdB protein of S. griseus was identical to the amino acid sequence deduced from the nucleotide sequence. The deduced amino acid sequence contains an "rpoD box" conserved in the principal sigma factors of eubacteria, and shows high similarity to the hrdB products of S. coelicolor A3(2) (89.9%) and S. aureofaciens (88.1%). The cloned gene encodes a principal sigma factor of S. griseus. The promoter region was identified by using a promoter-probe vector and by means of primer extension experiments. The transcription start point is located 158-bp upstream of the initintion codon.
1 This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. The nucleotide sequence reported in this paper has been deposited in the GenBank, EMBL, and DDBJ data base with the accession number L08071 [GenBank] .
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