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J. Biochem, 1995, Vol. 118, No. 3 500-507
© 1995 Japanese Biochemical Society

other

Opposite Actions of Transforming Growth Factor-ß1 on the Gene Expression of Atrial Natriuretic Peptide Biological and Clearance Receptors in a Murine Thymic Stromal Cell Line1

Takashi Agui*,2, Xiaonan Xin{dagger}, Yiqiang Cai{dagger}, Giljin Shim*, Youji Muramatsu{dagger}, Takahisa Yamada{dagger}, Hiromi Fujiwara{ddagger} and Kozo Matsumotoup{dagger}

*Institute for Experimental Animal Science, Nagoya City University Medical School Nagoya, Aichi 467
{dagger}Institute for Animal Experimentation, University of Tokushima School of Medicine Tokushima, Tokushima 770
{ddagger}Biomedical Research Center, Osaka University Medical School Suita, Osaka 565

2To whom correspondence should be addressed.

The regulation of the gene expression of the atrial natriuretic peptide receptor (ANPR)subtypes, ANPR-A, ANPR-B, and ANPR-C, was investigated in a murine thymic stromal cell line, MRL 104.8a. When MRL 104.8a cells were cultured with transforming growth factor (TGF)-ß1,[126I]ANP binding sites increased with increasing dose of TGF-ß. These binding sites were identified as ANPR-C by a displacement experiment with ANPR-C-specific ligand, C-ANF, and by the affinity cross-linking of the [125I] ANP binding sites with a chemical cross-linker to determine the molecular weight of the ANPR. This augmentation of the ANPR-C expression was elucidated to occur at the transcriptional level by Northern blot experiment, comparison of the relative amounts of mRNA by reverse transcription (RT)-PCR, and in vitro nuclear transcription assay. Conversely, the expression of the ANP biological receptors, ANPR-A and ANPR-B, was shown to be down-regulated by TGF-ß1. These data suggest that TGF-ß1regulates the gene expression of ANPRs in the thymic stromal cells and that ANP and TGF-ß1might affect the thymic stromal cell functions.

1This study was supported in part by the Uehara Memorial Foundation.


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