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J. Biochem, 1995, Vol. 118, No. 3 526-533
© 1995 Japanese Biochemical Society

other

Fast Atom Bombardment and Electrospray Ionization Tandem Mass Spectrometry of Sulfated Lewisx Trisaccharides1

Tadashi , II*,2, Yoko Ohashi*,3, Shigeki Nunomara{dagger}, Tomoya Ogawa{ddagger},§ and Yoshitaka Nagai*

*Glycobiology Research, Frontier Research Program
{ddagger}Laboratory of Synthetic Cellular Chemistry, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama 351-01
{dagger}Tokyo Research Institute, Nissin Food Products Corporation Kitano, Tokorozawa, Saitama 359
§Faculty of Agriculture, University of Tokyo Yayoi, Bunkyo-ku, Tokyo 113

2Present address: Research and Development Laboratories, Soda Aromatic Co., Ltd., Noda, Chiba 270-02.

3 To whom correspondence should be addressed.

Structural characterization of mono-, di-, and trisulfated triasaccharides by fast atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry is described. Both FAB and ESI collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of sulfated trisaccharides allowed identification of the sulfated sugar and provided information on the saccharide chain sequence. The ESI CID-MS/MS of multiple-charged ions provided even more structural information, and some of the useful daughter ions appeared in the higher mass region than the precursor owing to alower charge-state. These methodologies can be applied to the structural analysis of glycoconjugates with much larger molecular masses and higher polarity, such as the poly-sulfated analogs.

1This work was partially supported by a Grant-in-Aid for Scientific Research on Priority Areas (No. 06240247) from the Ministry of Education, Science and Culture of Japan.




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