J. Biochem, 1995, Vol. 118, No. 3 534-540
© 1995 Japanese Biochemical Society
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Induction of CD40 in Promyelocytic HL60 cells Cultured with Retinoic Acid and/or Various Cytokines1
The Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108
2 Present addresses: Department of Veterinary Public Health, School of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080
3 Department of Pediatrics, School of Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113.
4 To whom correspondence should be addressed.
The antigentic protein CD40 on the surface of Blymphocytes plays an important role in their Proliferation, immunoglobulin class switching, and rescue from apoptosis in the germinal center through interaction with T Lymphocytes expressing CD40 liganed. The protein is also found on the cell surface of other antigen-presenting cells such as monocytes, Dendritic cells, and thymic epithelium cells, but its presence in other myloid cells has not been reported. We show here that CD 40 protein in induced in promyelocytic HL60 cells, when cultured with retinoic acid, a vitamin that converts them to granulocyte-like cells. The cultured cells also expressed CD15, a Marker for granulocytes, and cytochrome an essential component of the superoxide-generating system in Phagocytes, on their surface. No detectable amount of mRNA for CD40 was found in naive HL60 cells, where as alarge amount of the message was induced in the cells cultured with the vitamin. Although CD40 expression was enhanced when the cells were further cultured with GM-CSF or IFN-
expression of CD14, a marker for Monocytes, was also enhanced. HL60 cells, therefore, express CD40 protein during differentiation not only toward monocytes but also toward granulocytes, at least transiently.
1 This investigation was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.