J. Biochem, 1995, Vol. 118, No. 3 601-606
© 1995 Japanese Biochemical Society
other |
Copy-Dependent and Position-Independent Expression of Rat Aldolase A Gene1
*Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565;
Department of Biochemistry, Saga Medical School Saga, Saga 849
Institute for Medical Genetics, Kumamoto University Medical School 4-24-1 Kuhonji, Kumamoto, Kumamoto 862
2 Present address: Division of Radiologicl Protection and Biology, National Institute of Health, 1-23-1 Toyma, Shinjuji, Tokyo 162.
3 To whom correspondence should be addressed. Phone: +81-6-833-5012 (Ext. 2506), Fax: +81-6-872-7485
In order to understand the molecular mechanisms of the temporal and spatial differences of gene expression in higher organisms, rat aldolase A gene carrying two distinct promoters was introduced into fertilized eggs and the resulting transgenic mice were analyzed. The transgene expression is tissue-specific and is developmentally regulated. In addition, the expression is regulated in a copy-dependent manner irrespective of where the transgene is integrated, suggesting that a mechanism excluding the effect of the integration site exists within the transgene itself. To explore the conformational change of this gene in the genome, the DNase I hypersensitive sites of the gene were examined. Three sites(DHS-1, 2, and 3) were identified upstream and downstream of the gene and these sites were retained in the transgene as well as in the gene observed endogenously.
1This work was supported in party by a Grant-in Aid from the Ministry of Education, Science and Culture of Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Voet, J. Vermeesch, A. Carens, J. Dürr, C. Labaere, H. Duhamel, G. David, and P. Marynen Efficient Male and Female Germline Transmission of a Human Chromosomal Vector in Mice Genome Res., January 1, 2001; 11(1): 124 - 136. [Abstract] [Full Text]
|
|