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J. Biochem, 1996, Vol. 119, No. 4 697-702
© 1996 Japanese Biochemical Society

research-article

Characterization and Biological Significance of Sialyl{alpha}2-3Galactosylß1-4Xylosylßl-(4-Methylumbelliferone) Synthesized in Cultured Human Skin Fibroblasts1

Keiichi Takagaki, Toshiya Nakamura, Shigeru Shibata, Tsuyoshi Higuchi and Masahiko Endo2

Department of Biochemistry, Hirosaki University School of Medicine 5 Zaifu-cho, Hirosaki 036

2 To whom correspondence should be addressed.

Human skin fibroblasts were incubated in the presence of a fluorogenic xyloside, 4-methyl-umbelliferyl-ß-D-xyloside (Xyl-MU), then the cultured medium was recovered, concentrated with a lyophilizer, and dialyzed against distilled water. The structures of the Xyl-MU derivatives purified from the dialyzable fraction were investigated. In addition to established glycosaminoglycans-MU (GAGs-MU), Gal-Gal-Xyl-MU, Gal-Xyl-MU, sul-phate-GlcA-Xyl-MU, GlcA-Xyl-MU, and Xyl-Xyl-MU, which were induced by Xyl-MU, an oligosaccharide having fluorescence was purified using a combination of gel filtration, ion-exchange chromatography and high-performance liquid chromatography, then subjected to carbohydrate composition analysis, enzyme digestion, Smith degradation, 1H-NMR, and ion-spray mass spectrometric analysis. From the data obtained, the oligosaccharide was considered to have the structure SA{alpha}2-3Galß1-4Xylßl-MU. The amount of MU-oligosaccharide in the cell culture increased with time and was dependent on the amount of Xyl-MU added. Its production was also different from that of Gal-Gal-Xyl-MU and Gal-Xyl-MU, which are biosynthetic intermediates of GAG-MU. Addition of CDP, an inhibitor of sialyltransferase, to the cell culture medium increased the secretion of GAG-MU. These results suggest that SA-Gal-Xyl-MU production may be related to the regulation of GAG-MU biosynthesis.

1 This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (Nos. 05274107, 05680518, and 60238285), and from the Karohji Medical Foundation, the Mizutani Foundation for Glycoscience, and the Nakatomi Foundation.


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