J. Biochem, 1996, Vol. 119, No. 4 749-767
© 1996 Japanese Biochemical Society
research-article |
A Catalogue of Genes in Mouse Embryonal Carcinoma F9 Cells Identified with Expressed Sequence Tags1
*Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565
Genome Information Research Center, Osaka University 3-1 Yamadaoka, Suita, Osaka 565
3 To whom all correspondence should be addressed. E-mail: shimada{at}biken.osaka-u.ac.jp
We used expressed sequence tags (ESTs) to identify genes expressed in mouse embryonal carcinoma F9 cells and prepared 2132 ESTs from undifferentiated F9 cDNA libraries: 1026 were prepared after randomly selecting clones from one of the libraries and the remaining 1106 ESTs were prepared after classifying 2896 clones of the libraries into four classes, according to the levels and patterns of expression. Among the former 1026 ESTs, 797 (78%) matched known genes, 61 (6%) matched database sequences of uncharacterized cDNAs, and 168 (16%) represented novel genes. The ESTs matching known genes were catalogued according to putative structural and cellular functions. As many as 53% were related to transcription and translation, and 19% were related to energy metabolism, including transcripts of mitochondrial DNA. These percentages were significantly higher in F9 cells than in the human heart and brain, and a human liver cell line, HepG2. We found that approximately 7% of the ESTs corresponding to low-abundance mRNAs are either related to retinoic acid-regulated genes or mammalian development-and/or differentiation-related genes. Cataloguing of the genes expressed in the F9 cells paves the way for isolating genes involved in early mammalian development.
1 This work was supported by a Grant-in-Aid for Creative Basic Research (06NP0401), and a Grant-in-Aid for Scientific Research (04454170) from the Ministry of Education, Science, Sports and Culture of Japan. A part of this work was also supported by a Grant for Aging from the Ministry of Health and Welfare of Japan, a Grant-in-Aid from the Japan Medical Association, and a Grant-in-Aid from the Uehara Memorial Foundation. The nucleotide sequence data reported in this paper have already been registered or will appear in the GSDB, DDBJ, EMBL, and NCBI nucleotide sequence databases, under the following accession numbers: D21355 [GenBank] to D21795 [GenBank] , D28603 [GenBank] to D28744 [GenBank] , and D76447 [GenBank] to D77996 [GenBank] .
2 Present address: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
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