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J. Biochem, 1996, Vol. 119, No. 4 811-816
© 1996 Japanese Biochemical Society

research-article

Characterization of a B. subtilis Minor Isoleucine tRNA Deduced from tDNA Having a Methionine Anticodon CAT1

Jitsuhiro Matsugi2, Katsutoshi Murao and Hisayuki Ishikura

Laboratory of Chemistry, Jichi Medical School 3311-1 Minamikawachi-machi, Tochigi 329-04

2To whom correspondence should be addressed. Phone:+ 81-285-44-2111 (Ext. 3366), Fax: +81-285-44-2858

Bacillus subtilis, which belongs to Gram-positive eubacteria, has been predicted to have a minor isoleucine tRNA transcribed from the gene possessing the CAT anticodon, which corresponds to methionine.We isolated this tRNA and determined its sequence including modified nucleotides. Modified nucleotide analyses using TLC,UV,and FAB mass spectros-copy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-ß-D-ribofuranosyl pyrimidine]. As a result, this tRNA agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA.

1The nucleotide sequence data of a B. subtilis minor tRNA110 reported in thia paper will appear in the GSDB, DDBJ, EMBL,and NCBI nucleotide sequence databases with the accession number D26446 [GenBank] .


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