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J. Biochem, 1997, Vol. 121, No. 4 633-636
© 1997 Japanese Biochemical Society

other

Real Time Observation of Binding of Herpes Simplex Virus Type 1 (HSV-1) to Vero Cells and Neutralization of HSV-1 by Sulfonated Human Immunoglobulin

Kazuko Inoue*, Toshihiko Arai*,1 and Masaaki Aoyagi{dagger}

*Department of Microbiology, Meiji College of Pharmacy 1-35-23 Nozawa, Setagaya-ku, Tokyo 154
{dagger}Scientific Instruments Laboratory, Nissei Sangyo Co., Ltd. Atsugi, Kanagawa 243-01

1To whom correspondence should be addressed. Phone: +81-3-3424-8616, Fax: +81-3-3795-7525

A real time biomolecular interaction assay system involving an optical sensor was applied to the quantitative analysis of the binding of herpes simplex virus type 1 (HSV-1) to Vero cells, and the neutralization antibody titer against this virus with a commercially available sulfonated human immunoglobulin preparation. The virus titers in a viral solution and the neutralizing antibody titer in the human immunoglobulin preparation could be successfully estimated in a short time with this system without any difficult cytopathic effect analysis of cultured cells.


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