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J. Biochem, 1997, Vol. 121, No. 4 671-676
© 1997 Japanese Biochemical Society


research-article

Structure of the Ascidian, Halocynthia roretzi, Troponin C Gene1

Hajime Julie Yuasa, Shigeru Sato, Hiroaki Yamamoto and Takashi Takagi2

Biological Institute, Graduate School of Science, Tohoku University Aoba-ku, Sendai, Miyagi 980-77

2To whom correspondence should be addressed. Tel: +81-22-217-6677, Fax: +81-22-263-9206 E-mail: a21692{at}cctu.cc.tohoku.ac.jp

Two distinct cDNAs encoding troponin C (TnC) isoforms were isolated from the ascidian, Halocynthia roretzi One is expressed in adult body wall smooth muscle and heart muscle, and the other in larval striated muscle. The H. rorezti gene is composed of 7 exons separated by 6 introns, and Southern blot analysis showed that TnC is a single copy gene product. The two isoforms of TnC were derived through the alternative splicing of the third exon.

1The nucleotide sequences have been submitted to the DDBJ under the accession numbers, D88494 [GenBank] (H. roretzi body wall muscle TnC cDNA), D88495 [GenBank] (H. rorezi larval TnC cDNA), and D88496 [GenBank] (H. roretzi TnC genome).


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