J. Biochem, 1998, Vol. 123, No. 1 101-106
© 1998 Japanese Biochemical Society
research-article |
Integrin-Associated Protein (IAP, Also Termed CD47) Is Involved in Stroma-Supported Erythropoiesis1
*Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University Seiryomachi, Aoba-ku, Sendai 980-77
Laboratory of Cellular Biosynthesis, Institute of Molecular and Cellular Biosciences, The University of Tokyo Yayoi 1-1-1, Bunkyo-ku, Tokyo 113
2To whom correspondence should be addressed. Tel: +81-22-717-8483, FAX: -81-22-717-8488, E-mail: mobinata{at}idac.tohoku.ac.jp
Erythropoiesis is regulated by the hematopoietic microenvironment of the spleen, fetal liver, and bone marrow in mice. We previously showed that established stromal cells from these organs selectively support erythropoiesis in vitro. To identify the cell surface molecule(s) on the stromal cells involved in erythropoiesis, we raised monoclonal antibodies (MAbs) to MSS62 stromal cells derived from newborn spleen and obtained MAb100.1, which partially inhibited the stroma-supported erythropoiesis in vitro. Using an expression cDNA library of MSS62 cells, we cloned a gene encoding the protein recognized by MAb100.1 and identified it as integrin-associated protein (LAP, also termed CD47), which may play a general role in integrin-mediated signal transduction. IAP/CD47 is expressed in the stromal cells of spleen, fetal liver, and bone marrow, and in a variety of hematopoietic cells including erythroblasts. Thus, IAP may be partly involved in the erythropoietic supporting ability of the stromal cells.
1This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan.
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