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J. Biochem, 1998, Vol. 123, No. 1 120-127
© 1998 Japanese Biochemical Society

research-article

Ku Antigen Binds to Alu Family DNA1

Takayuki Tsuchiya*, Yumiko Saėgusa*, Takahiro Taira*, Tsuneyo Mimori{dagger}, Sanae M.M. Iguchi-Ariga{ddagger} and Hiroyoshi Ariga*,2

*Faculty of Pharmaceutical Sciences, Hokkaido University Kita 12, Nishi 6, Kita-ku, Sapporo 060
{ddagger}College of Medical Technology, Hokkaido University Kita 12, Nishi 6, Kita-ku, Sapporo 060
{dagger}Department of Internal Medicine, Keio University School of Medicine 35 Shinanomachi, Shinjuku-ku, Tokyo 160

2To whom correspondence should be addressed. Tel: +81-11-706-3745, Fax: +81-11-706-4988, E-mail: hiro{at}pharm.hokudai.ac.jp

The GC-rich segment containing GGAGGC (Alu core) is conserved within the RNA polymerase III (pol III) promoters of Alu family sequences. We have shown that the GGAGGC motif functions as a modulator of DNA replication as well as of transcription, and identified the proteins binding to the motif in human HeLa cells. In this study, the Alu core binding proteins were partially purified from human Raji cells by using an Alu core DNA affinity column. Both the proteins thus purified were implied to be subunits of Ku antigen based on the following criteria: The molecular weights of the proteins estimated on gel electrophoreses were 70 and 85 kDa, respectively, under denaturing conditions, while under non-denaturing conditions only one band was observed for the same sample at 150 kDa, probably representing hetero-dimer formed between the 70 and 85 kDa proteins. The sizes and the hetero-dimer formation are reminiscent of the 70 and 80 kDa subunits of Ku antigen (Ku-p70 and Ku-p80). Moreover, the purified proteins were immunoreactive with anti-Ku antibodies, and the specific DNA-protein complex on the Alu core element was cancelled by the anti-Ku antibodies. The nucleoprotein complex showed the same clipping patterns as those of the complex between the Alu core element and an authentically purified Ku antigen after proteolytic cleavage with trypsin and chymotrypsin.

1This work was supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan, and the Terumo Life Science Foundation.


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