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J. Biochem, 1998, Vol. 123, No. 1 87-93
© 1998 Japanese Biochemical Society

research-article

High Pressure Induces G2 Arrest in Murine Erythroleukemia Cells1

Masaki Matsumoto*,2, Takeo Yamaguchi*, Yasuyuki Fukumaki{dagger}, Reiko Yasunaga* and Shigeyuki Terada*

* Department of Chemistry, Faculty of Science, Fukuoka University 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-80
{dagger} Institute of Genetic Information, Kyushu University 3-1-1 Maidashi, Higashi-ku, Fukuoka 812

2To whom correspondence should be addressed.

The effect of high pressure on proliferation and cell cycle progression was examined using murine erythroleukemia (MEL) cells. The MEL cells were exposed to high pressures (0.1–130 MPa) and then cultured for 5 days at atmospheric pressure. The proliferation of MEL cells was unaffected up to 60 MPa, but was suppressed at 80–110 MPa. Above 120 MPa, the cells were fragmented. The cell cycle analysis of 80 MPa-treated MEL cells showed that the cells in S phase are most sensitive to high pressure and they arrest in G2 phase. Interestingly, G2-arrested cells reinitiated DNA synthesis, resulting in giant cells with high DNA contents. Furthermore, when such G2-arrested cells were exposed to caffeine, premature mitosis, characterized by chromosome pulverization, was observed. These results suggest that the suppression of proliferation in high-pressure-treated MEL cells is associated with G2 arrest following S phase delay. Thus, it seems valuable to apply high pressure to the investigation of the cell cycle.

1This work was partly supported by a grant from the Central Research Institute of Fukuoka University.


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