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J. Biochem, 1998, Vol. 123, No. 6 1031-1035
© 1998 Japanese Biochemical Society

research-article

Existence of Two Isoforms of Extracellular Signal-Regulated Kinase in Fish1

Hisashi Hashimoto*, Yoshihiro Yokoyama{dagger}, Yoshiyuki Matsuo*, Haruhiko Toyohara*,2, Michiaki Kohno{ddagger} and Morihiko Sakaguchi*

*Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-8502
{dagger}Interdisciplinary Research Institute of Bioscience, Mukogawa Women's University 6-46, Ikebiraki, Nishinomiya, Hyogo 663-8558
{ddagger}Laboratory of Cell Regulation, School of Pharmaceutical Sciences, Nagasaki University 1-14, Bunkyo-machi, Nagasaki 852-8131

2To whom correspondence should be addressed. Tel: +81-75-753-6446, Fax: +81-75-753-6446, E-mail: toyohara{at}kais.kyoto-u.ac.jp

Full-length cDNAs for extracellular signal-regulated kinases (ERK1 and ERK2) were isolated from a carp ovary cDNA library. The deduced amino acid sequences of carp ERK1 (cERK1) and ERK2 (cERK2) exhibited high degrees of homology to the known sequences of the ERK group. Northern blot analysis showed that cERK1 mRNA was not expressed in a tissue-specific manner, though the level of expression of cERK2 mRNA varied among tissues. Western blot analysis of the brain, kidney, and ovary confirmed the expression of cERK1 and cERK2 in carp. Our findings indicate that two isoforms of ERK, ERK1 and ERK2, exist in fish.

1This study was supported in part by a grant (97L00902) from the Research for the Future Program of the Japan Society for the Promotion of Science. The DDBJ, GenBank, and EMBL accession numbers for cERK1 and cERK2 are AB006038 [GenBank] and AB006039 [GenBank] , respectively.


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