J. Biochem, 1998, Vol. 123, No. 6 1048-1054
© 1998 Japanese Biochemical Society
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Two Distinct Upstream Regions Are Involved in Expression of the Catalase Gene in Schizosaccharomyces pombe in Response to Oxidative Stress
Department of Genetics, Institute for Developmental Research Kagiya-cho, Kasugai, Aichi 480-0392
1To whom correspondence should be addressed.
The DNA region responsible for the induction of the catalase gene of Schizosaccharomyces pombe in response to oxidative stress was determined by constructing a series of deletions in the 5'-flanking region of the gene. Cells having deletion –672 (numbered with the transcription start site as +1) to –111 showed no significant difference in catalase expression from the wild-type cells. Cells having deletion –672 to –89 showed reduced basal expression of the catalase mRNA, but retained the ability of induction in response to oxidative stress. Cells having deletion –672 to –55 completely lost the ability to express the catalase mRNA. These results suggested that two regions, –89 to –55 and –111 to –89, are involved in expression of the catalase gene. The DNA region of –89 to –55 overlapped with the Atfl binding sequence. The Atfl is a bZIP transcription factor with an important role in stress response under the control of the Spcl mitogen activated protein (MAP) kinase. Introduction of the atfl– or spcl– mutation into the mutant having a deletion in –672 to –89 completely abolished the expression of the catalase mRNA. This result indicated that the Spcl-Atfl cascade is involved in expression of the catalase gene through the region of –89 to –55. In mutants spcl– and atfl–, basal expression and induction by hydrogen peroxide of catalase mRNA were observed. These results revealed that not only the Atfl binding site but also another DNA element independent of the Spcl-Atfl pathway is involved in the expression of the catalase gene in response to oxidative stress in S. pombe. Proteins that bound specifically to each DNA element existed in the cell extract of the wild-type S. pombe.
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