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J. Biochem, 2001, Vol. 129, No. 1 133-138
© 2001 Japanese Biochemical Society

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Osteoblast-Related Gene Expression of Bone Marrow Cells during the Osteoblastic Differentiation Induced by Type I Collagen

Morimichi Mizuno1 and Yoshinori Kuboki

Department of Oral Health Science, School of Dentistry, Hokkaido University 060-8586 Sapporo

1To whom correspondence should be addressed. Tel/Fax: +81-11-706-4235, E-mail: mmizuno{at}den.hokudai.ac.jp

Bone marrow contains multipotent cells that differentiate into fibroblasts, adipocytes, and osteoblasts. Recently we found that type I collagen matrix induced the osteoblastic differentiation of bone marrow cells. Three weeks after cells were cultured with type I collagen, they formed mineralized tissues. In this study, we investigated the expression of osteoblast-related genes (alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin, and cbfa-1) during the osteoblastic differentiation. The expression of alkaline phosphatase and osteopontin genes increased time-dependently during the osteoblastic differentiation. Osteocalcin and bone sialoprotein genes were expressed in cells that formed mineralized tissues, and both were expressed only after cells reached the mineralized tissue-formation stage. On the other hand, the cbfa-1 gene was expressed from the early differentiation stage. The Asp-Gly-Glu-Ala (DGEA) amino acid domain of type I collagen interacts with the {alpha}2ß1 integrin receptor on the cell membrane and mediates extracellular signals into cells. When the collagen-integrin interaction was interrupted by the addition of DGEA peptide to the culture, the expression of osteoblastic phenotypes of bone marrow cells was inhibited. These findings imply that the collagen-{alpha}2ß1 integrin interaction is an important signal for the osteoblastic differentiation of bone marrow cells.


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