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J. Biochem, 2001, Vol. 129, No. 1 69-76
© 2001 Japanese Biochemical Society

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Hepatic Fatty Acid-Binding Proteins of a Teleost, Lateolabrax japonicus. The Primary Structures and Location of a Disulfide Bond1

Shoji Odani*,2, Katsuya Baba*, Yutaka Tsuchida*, Yutaka Aoyagi{dagger}, Shuji Wakui{ddagger} and Yoshiaki Takahashi§

*Department of Biology, Faculty of Science, Niigata University Ikarashi, Niigata 950-2181
{dagger}Department of Internal Medicine III, School of Medicine, Faculty of Medicine, Niigata University Asahimachi, Niigata 951-8122
{ddagger}Department of Physiology, School of Medicine, Faculty of Medicine, Niigata University Asahimachi, Niigata 951-8122
§Department of Medical Technology, School of Health Science, Faculty of Medicine, Niigata University Asahimachi, Niigata 951-8122

2To whom correspondence should be addressed. Tel: +81-25-262-6174, Fax: +81-25-262-6116, E-mail: sodani{at}sc.niigata-u.ac.jp

Two fatty acid-binding proteins (FABP), FABP-1 and FABP-2, were purified from the liver cytosol of the teleost, Lateolabrax japonicus (Japan sea bass), and characterized. The complete primary structure of FABP-2 was determined by protein analysis to be the following: MDFSGTWQVY AQENYEEFLR AMELPADVIK MAKDIKPITE IKQSGNDFVV TSKTPGKTVT NSFTIGKEAD ITTMDGKKIR CVVNLEGGKL VCNTGKFCHI QELRGGEMVE TLTMGSTTLI RKSKKM. Partial peptide sequences of FABP-1 were also determined. Phylogenetic analysis indicates that FABP-2 is a homologue of mammalian hepatic FABP, whereas FABP-1 is most similar to the members of mammalian cardiac FABP subfamily. L. japonicus FABP-2 contains three cysteine residues, and a disulfide bond is identified between Cys-81 and Cys-92. A theoretical model of FABP-2 generated by a homology modeling method indicates close proximity of the two cysteine residues in the three-dimensional structure. This is a rather rare case of cytosolic protein having a disulfide bond under the normally reducing conditions of the cytosol, though the presence or absence of disulfide bonds does not seem to affect the ligand-binding ability.

1This research was supported by a Grant-in-Aid for Scientific Research (C-10680579) from the Ministry of Education, Science, Sports and Culture of Japan.


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