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J. Biochem, 2003, Vol. 133, No. 2 247-251
© 2003 Japanese Biochemical Society

CELL

Integration of Cytochrome b5 into Endoplasmic Reticulum Membrane: Participation of Carboxy-Terminal Portion of the Transmembrane Domain

Satoshi Tanaka+, Jun-ya Kinoshita, Rieko Kuroda§, and Akio Ito,

Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka 812-8581

Integration of cytochrome b5 (b5), a tail-anchored protein located in the endoplasmic reticulum (ER) membrane, into the membrane was studied. Mutation of three amino acids, -Leu-Met-Tyr, at the carboxy-terminal end of the transmembrane segment of b5 to alanines resulted in localization of the mutated protein, b5LMY/AAA, in the cytosol as well as in the ER membrane. When an N-glycosylation site was introduced at the carboxy-terminal end of b5LMY/AAA, a substantial amount of the glycosylated form of the mutant protein was recovered in the cytosol fraction. A portion of the mutant protein recovered in the ER was released from the membrane by incubation with the cytosol fraction, but no further release was observed in the second incubation, suggesting that b5 is present in two different states, loosely-bound and firmly-integrated forms, in the ER membrane. These results suggest that b5 is integrated into the ER membrane via the loosely bound state, in which the carboxy-terminal end of the molecule is inserted into the luminal side of the vesicle but is easily translocated back to the cytosol, and that the three amino acids are important for conversion of the loosely-bound state to the firmly-integrated state.

+ Daikoku Branch office, Yokohama Customs, Yokohama 230-0054.

§ Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Fukuoka 803-0814.

To whom Correspondence should be addressed. Tel/Fax: +81-92-642-2530, E-mail: a.itoscc{at}mbox.nc.kyushu-u.ac.jp


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