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J. Biochem, 2003, Vol. 133, No. 4 461-465
© 2003 Japanese Biochemical Society

BIOCHEMISTRY

Hybrid Respiration in the Denitrifying Mitochondria of Fusarium oxysporum

Naoki Takaya1, Seigo Kuwazaki1, Yoshiaki Adachi1, Sawako Suzuki1, Tomoko Kikuchi1, Hiro Nakamura2, Yoshitsugu Shiro2 and Hirofumi Shoun+,3

1 Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572; 2 The Institute of Physical and Chemical Research Institute (RIKEN), RIKEN Harima Institute, Mikazuki-Cho, Sayo, Hyogo 679-5143; and 3 Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657

Induction of the mitochondrial nitrate-respiration (denitrification) system of the fungus Fusarium oxysporum requires the supply of low levels of oxygen (O2). Here we show that O2 and nitrate (NO3–) respiration function simultaneously in the mitochondria of fungal cells incubated under hypoxic, denitrifying conditions in which both O2 and NO3– act as the terminal electron acceptors. The NO3– and nitrite (NO2–) reductases involved in fungal denitrification share the mitochondrial respiratory chain with cytochrome oxidase. F. oxysporum cytochrome c549 can serve as an electron donor for both NO2– reductase and cytochrome oxidase. We are the first to demonstrate hybrid respiration in respiring eukaryotic mitochondria.

+ To whom correspondence should be addressed. Tel/Fax: +81-35841-5148, E-mail: ahshoun{at}mail.ecc.u-tokyo.ac.jp


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