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J. Biochem, 2003, Vol. 133, No. 5 571-576
© 2003 Japanese Biochemical Society

BIOCHEMISTRY

Inhibitory Effects of Green Tea Catechins on the Activity of Human Matrix Metalloproteinase 7 (Matrilysin)

Hiroshi Oneda, Misa Shiihara and Kuniyo Inouye+

Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502

Inhibitory effects of green tea catechins and their derivatives on the matrilysin-catalyzed hydrolysis of a synthetic substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH2 [MOCAc-PLGL(Dpa)AR], were examined. The 10 catechins examined were classified into three groups according to their inhibition potency. Catechins with a galloyl group at the 3 position, including a major component of green tea catechin, (–)-epigallo-3-catechin gallate [(–)-EGCG], were the most potent inhibitors and inhibited matrilysin in a non-competitive manner with Ki values of 0.47–1.65 µM. The inhibitory potency of (–)-EGCG was not influenced by the presence of an inhibitor, ZnCl2, suggesting that the inhibitions of matrilysin by (–)-EGCG and by ZnCl2 might be independent of each other. The inhibitory effects of green tea catechins suggest that a high intake of green tea might be effective for the prevention of tumor metastasis and invasion in which matrilysin is concerned.

+ To whom correspondence should be addressed: Tel: +81-75-753-6266, Fax: +81-75-753-6265, E-mail: inouye{at}kais.kyoto-u.ac.jp


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