Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Numazawa, S.
Right arrow Articles by Yoshida, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Numazawa, S.
Right arrow Articles by Yoshida, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 2003, Vol. 133, No. 5 599-605
© 2003 Japanese Biochemical Society

BIOCHEMISTRY

Regulation of ERK-Mediated Signal Transduction by p38 MAP Kinase in Human Monocytic THP-1 Cells

Satoshi Numazawa+, Masahiko Watabe, Satoshi Nishimura, Masahiro Kurosawa, Makoto Izuno and Takemi Yoshida

Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555

SB 203580 has been widely used to specifically shut down the p38 MAP kinase-dependent pathway, although it is capable of inducing c-Raf kinase activity in cells. The present study demonstrates that SB 203580 activates members of the ERK cascade, c-Raf, MEK, and ERK, in human monocytic THP-1 cells. The activation of these kinases was sustained for at least 24 h after SB 203580 treatment and was also observed in U937 cells, suggesting that c-Raf efficiently transduces the signal even in the presence of the inhibitor in these cells. However, the expression of ERK cascade–dependent genes, such as c-fos and IL-1ß, was extremely limited. Analysis of the cellular distribution of ERK in SB 203580-treated cells indicated that nuclear translocation of phosphorylated ERK was impaired. Also, nuclear translocation of ERK induced by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was inhibited by SB 239063, which does not associate with c-Raf and is highly selective for p38 MAP kinase. In addition, the forced expression of the dominant negative mutant of p38 MAP kinase suppressed serum responsive element-dependent transactivation induced by TPA. These results suggest that the steady-state level of p38 MAP kinase activity modulates ERK signaling.

+ To whom correspondence should be addressed: Tel: +81-3-3784-8206, Fax: +81-3-3784-8246, E-mail: numazawa{at}pharm.showa-u.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. H. Pillinger, N. Marjanovic, S.-Y. Kim, Y.-C. Lee, J. U. Scher, J. Roper, A. M. Abeles, P. I. Izmirly, M. Axelrod, M. Y. Pillinger, et al.
Helicobacter pylori Stimulates Gastric Epithelial Cell MMP-1 Secretion via CagA-dependent and -independent ERK Activation
J. Biol. Chem., June 29, 2007; 282(26): 18722 - 18731.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Wang, J. Zhang, S. Theel, J. J. Barb, P. J. Munson, and R. L. Danner
Nitric oxide activation of Erk1/2 regulates the stability and translation of mRNA transcripts containing CU-rich elements
Nucleic Acids Res., June 6, 2006; 34(10): 3044 - 3056.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
O. Kogkopoulou, E. Tzakos, G. Mavrothalassitis, C. T. Baldari, F. Paliogianni, H. A. Young, and G. Thyphronitis
Conditional up-regulation of IL-2 production by p38 MAPK inactivation is mediated by increased Erk1/2 activity
J. Leukoc. Biol., May 1, 2006; 79(5): 1052 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
H. H. Kim, C. M. Shin, C.-H. Park, K. H. Kim, K. H. Cho, H. C. Eun, and J. H. Chung
Eicosapentaenoic acid inhibits UV-induced MMP-1 expression in human dermal fibroblasts
J. Lipid Res., August 1, 2005; 46(8): 1712 - 1720.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. G. Munshi, Y. I. Wu, S. Mukhopadhyay, A. J. Ottaviano, A. Sassano, J. E. Koblinski, L. C. Platanias, and M. S. Stack
Differential Regulation of Membrane Type 1-Matrix Metalloproteinase Activity by ERK 1/2- and p38 MAPK-modulated Tissue Inhibitor of Metalloproteinases 2 Expression Controls Transforming Growth Factor-{beta}1-induced Pericellular Collagenolysis
J. Biol. Chem., September 10, 2004; 279(37): 39042 - 39050.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Ohkusu-Tsukada, N. Tominaga, H. Udono, and K. Yui
Regulation of the Maintenance of Peripheral T-Cell Anergy by TAB1-Mediated p38{alpha} Activation
Mol. Cell. Biol., August 15, 2004; 24(16): 6957 - 6966.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Mukhopadhyay, H. G. Munshi, S. Kambhampati, A. Sassano, L. C. Platanias, and M. S. Stack
Calcium-induced Matrix Metalloproteinase 9 Gene Expression Is Differentially Regulated by ERK1/2 and p38 MAPK in Oral Keratinocytes and Oral Squamous Cell Carcinoma
J. Biol. Chem., August 6, 2004; 279(32): 33139 - 33146.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.