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J. Biochem, 2003, Vol. 133, No. 6 713-718
© 2003 Japanese Biochemical Society

MOLECULAR BIOLOGY

HCC-Associated Protein HCAP1, a Variant of GEMIN4, Interacts with Zinc-Finger Proteins

Yujun Di1, Jinjun Li2, Yu Zhang1, Xianghuo He2, Hong Lu1, Dongbin Xu1, Jiqiang Ling1, Keke Huo1, Dafang Wan2, Yu-Yang Li1,+ and Jianren Gu2

1 State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People’s Republic of China; and 2 State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032 People’s Republic of China

The gene HCAP1 (HCC-associated Protein 1), one variant of GEMIN4, has been mapped in a minimum LOH region on chromosome 17p13.3 and encodes a 1047-amino acid protein. Function predictions based on the amino acid sequence of protein HCAP1 revealed it to contain one helix-loop-helix motif and one leucine zipper domain. Using yeast two-hybrid screening, five zinc-finger proteins were identified as HCAP1-interacting proteins. Among them, NDP52 (nuclear dot protein 52) appeared most frequently in positive clones and was the most strongly interacting protein. Then, the interaction between HCAP1 and NDP52 was confirmed by GST pull-down assay and a coimmunoprecipitation experiment. Moreover, an immunofluorescent staining assay indicated that NDP52 colocalizes with HCAP1 in the cytoplasm. By deletion analysis, the leucine zipper domain of HCAP1 and the zinc finger domain of NDP52 were identified as important regions responsible for the interaction.

+ To whom correspondence should be addressed. Tel/Fax: +86-21-65643436, E-mail: liyuy{at}online.sh.cn


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