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J. Biochem, 2003, Vol. 134, No. 1 51-55
© 2003 Japanese Biochemical Society

BIOTECHNOLOGY

Conversion of Pyridylamino Sugar Chains to Corresponding Reducing Sugar Chains

Chiaki Takahashi, Shin-ichi Nakakita and Sumihiro Hase+,

Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043

Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J. Biochem. 112, 266–268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet reaction. The reaction conditions were optimized so as to obtain the maximal product yield using 1-amino-1-deoxylactose and 1-amino-1-deoxy-N-acetylglucosamine. When the established procedure was successively applied to pyridylamino high-mannose and complex-type sugar chains, the corresponding reducing sugar chains were obtained in yields of 30%.

+ To whom correspondence should be addressed. Tel: +81-6-6850-5380, Fax: +81-6-6850-5383.


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