Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Fukumura, J.
Right arrow Articles by Nishimoto, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukumura, J.
Right arrow Articles by Nishimoto, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 2003, Vol. 134, No. 1 71-82
© 2003 Japanese Biochemical Society

CELL

A Temperature-Sensitive Mutant of the Mammalian RNA Helicase, DEAD-BOX X Isoform, DBX, Defective in the Transition from G1 to S Phase

Junko Fukumura, Eishi Noguchi+,, Takeshi Sekiguchi and Takeharu Nishimoto§

Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka 812-8582

ts ET24 cells are a novel temperature-sensitive (ts) mutant for cell proliferation of hamster BHK21 cells. The human genomic DNA which rescued the temperature-sensitive lethality of ts ET24 cells was isolated and screened for an open reading frame in the deposited human genomic library. X chromosomal DBX gene encoding the RNA helicase, DEAD-BOX X isoform, which is homologous to yeast Ded1p, was found to be defective in this mutant. The single point mutation (P267S) was localized between the Motifs I and Ia of the hamster DBX of ts ET24 cells. At the nonpermissive temperature of 39.5°C, ts ET24 cells were arrested in the G1-phase and survived for more than 3 days. In ts ET24 cells, total protein synthesis was not reduced at 39.5°C for 24 h, while mRNA accumulated in the nucleus after incubation at 39.5°C for 17 h. The amount of cyclin A mRNA decreased in ts ET24 cells within 4 h after the temperature shift to 39.5°C, consistent with the fact that the entry into the S-phase was delayed by the temperature shift.

+ Present address: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037, USA.

§ To whom correspondence should be addressed. Tel: +81-92-642-6175, Fax: +81-92-642-6183, E-mail: tnishi{at}molbiol.med.kyushu-u.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
M.-C. Lai, Y.-H. W. Lee, and W.-Y. Tarn
The DEAD-Box RNA Helicase DDX3 Associates with Export Messenger Ribonucleoproteins as well asTip-associated Protein and Participates in Translational Control
Mol. Biol. Cell, September 1, 2008; 19(9): 3847 - 3858.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
H.J. Ditton, J. Zimmer, C. Kamp, E. Rajpert-De Meyts, and P.H. Vogt
The AZFa gene DBY (DDX3Y) is widely transcribed but the protein is limited to the male germ cells by translation control
Hum. Mol. Genet., October 1, 2004; 13(19): 2333 - 2341.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.