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J. Biochem, 2003, Vol. 134, No. 3 459-465
© 2003 Japanese Biochemical Society

BIOCHEMISTRY

Minimally Modified LDL Is an Oxidized LDL Enriched with Oxidized Phosphatidylcholines

Hiroyuki Itabe*, Masahiro Mori, Yasuyuki Fujimoto, Yusuke Higashi and Tatsuya Takano

Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, 1091-1 Suarashi, Sagamiko, Tsukui, Kanagawa 199-0195

The oxidative modification of low-density lipoprotein (LDL) is involved in atherogenesis. Among a variety of modified LDLs mentioned in the literature, so-called minimally modified LDL (MM-LDL) was reported to have pro-atherogenic properties despite minimal changes in its oxidative measures. After treatment of LDL with 1 µM FeSO4 at 4°C for 96 h, the resulting MM-LDL showed a slight increase in thiobarbituric acid-reactive substances (TBARS) and little association with macrophages. On the other hand, heavily oxidized LDL, which was prepared by copper-induced oxidation of LDL at 37°C, showed a sharp increase in TBARS and strong association with macrophages. By introducing a fluorometric procedure to detect aldehyde-containing phosphatidylcholines (aldehyde-PCs), we examined the amounts of aldehyde-PCs in modified LDL preparations. Aldehyde-PCs increased to 23.4 pmol/µg protein in MM-LDL, which was more than four-fold higher than in the heavily oxidized LDL. We conclude that MM-LDL is a unique type of oxidized LDL enriched with aldehyde-PCs.

* To whom correspondence should be addressed. Fax: +81-426-85-3738, E-mail: h-itabe{at}pharm.teikyo-u.ac.jp


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