Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Fujinoki, M.
Right arrow Articles by Okuno, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujinoki, M.
Right arrow Articles by Okuno, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 2003, Vol. 134, No. 4 559-565
© 2003 Japanese Biochemical Society

CELL

Identification of the 58-kDa Phosphoprotein Associated with Motility Initiation of Hamster Spermatozoa

Masakatsu Fujinoki*,1, Takeshi Kawamura2, Toshifusa Toda3, Hideki Ohtake1, Nobuyoshi Shimizu2, Sadao Yamaoka1 and Makoto Okuno4

1 Department of Physiology, Dokkyo University School of Medicine, Mibu, Tochigi 321-0293; 2 Department of Molecular Biology, Keio University School of Medicine, Shinanomachi, Shinjuku-ku, Tokyo 160-8582; 3 TMIG Proteomics Collaboration Center, Tokyo Metropolitan Institute of Gerontology, Sakaecho, Itabashi-ku, Tokyo 173-0015; and 4 Department of Biology, Graduate School of Arts and Science, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902

In our previous paper [M. Fujinoki et al. (2001) Biomed. Res. 22, 45–58], we reported that the 58-kDa protein obtained from hamster sperm flagella was phosphorylated at serine residues in association with the start of motility. In the present experiments, we identified and localized the 58-kDa protein. The 58-kDa protein was assumed to exist in the acrosomal region domain of the sperm head and the whole sperm flagellum. In particular, a large amount of 58-kDa protein was localized in the equatorial segment of the acrosomal region domain of the sperm head and the middle piece of the sperm flagellum. In the next step, the 58-kDa protein was identified by peptide mass finger printing and LC-MS/MS analysis. The results suggested that the 58-kDa protein was ATP synthase H+ transporting F1 ß, which is one of the mitochondrial components. Therefore, it is likely that the 58-kDa protein is associated with ATP production in the mitochondrial sheath in the middle piece of the sperm flagellum, and H+ transport in the sperm head and the sperm flagellum except for the middle piece, since ATP synthase also acts as an H+ pump.

* To whom correspondence should be addressed. Tel: +81-282-87-2125, Fax: +81-282-86-7835, E-mail: fujinoki{at}dokkyomed.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
V. Kumar, N. Rangaraj, and S. Shivaji
Activity of Pyruvate Dehydrogenase A (PDHA) in Hamster Spermatozoa Correlates Positively with Hyperactivation and Is Associated with Sperm Capacitation
Biol Reprod, November 1, 2006; 75(5): 767 - 777.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.