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J. Biochem, 2003, Vol. 134, No. 5 637-639
© 2003 Japanese Biochemical Society

BIOCHEMISTRY

Role of the Zinc Fingers of HIV-1 Nucleocapsid Protein in Maturation of Genomic RNA

Seiki Baba1, Ken-ichi Takahashi*,1, Yoshio Koyanagi2, Naoki Yamamoto3, Hiroshi Takaku1, Robert J. Gorelick4 and Gota Kawai§,1

1 Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino-shi, Chiba 275-0016; 2 Department of Virology, Tohoku University School of Medicine, 2-1 Seiryou-machi, Aoba-ku, Sendai 980-8575; 3 Department of Molecular Virology, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519; and 4 dAIDS Vaccine Program, SAIC Frederick, Inc., NCI at Frederick, Frederick, Maryland 21702, USA

The nucleocapsid protein of HIV-1 consists of two basic amino acid regions and two zinc fingers. We investigated the requirement of these domains for the structural conversion of a 39mer RNA covering the dimerization initiation site by using three peptides; wild-type NCp7, a mutant in which the two zinc fingers are mutated, and another mutant in which the two zinc fingers are deleted. The two mutants exhibited similar conversion activities to each other, which were lower than that of the wild-type, indicating that the two basic regions exhibit some activity for RNA chaperone, as we suggested before, and the zinc fingers enhance the efficiency of this activity.

* Present address: Department of Bioscience, Faculty of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama, Shiga 526-0829.

§ To whom correspondence should be addressed. Tel/Fax: +81-47-478-0425, E-mail: gkawai{at}ic.it-chiba.ac.jp


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