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J. Biochem, 2004, Vol. 135, No. 2 171-177
© 2004 The Japanese Biochemical Society

CELL

Binding between Azurocidin and Calreticulin: Its Involvement in the Activation of Peripheral Monocytes

Yukiko Okuyama1,2, Jang-hyun Cho1, Yuki Nakajima1, Ko-ichi Homma2, Kazuhisa Sekimizu2 and Shunji Natori*,1

1 Natori Special Laboratory, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198; and 2 Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0033

We found that azurocidin, a secretory protein in neutrophils, binds to calreticulin, a multifunctional chaperone of the endoplasmic reticulum. Azurocidin is known to induce cytokine production in monocytes, but the mechanism of monocyte activation by azurocidin remains unknown. On the other hand, an antibacterial peptide, KLKLLLLLKLK-NH2 (L5), is known to bind to cell surface calreticulin of human neutrophils, resulting in their activation to produce O2–. Therefore, we examined whether cell surface calreticulin is involved in the activation of human monocytes by azurocidin to produce IL-6. We found that carlreticulin is in fact located on the surface of monocytes and that the IL-6 production stimulated by an azurucidin is inhibited by anti-calreticulin antibody. Possibly, binding between cell surface calreticulin and azurocidin is prerequisite for the activation of monocytes by azurocidin to produce IL-6.

* To whom correspondence should be addressed. Tel: +81-48-467-9438, Fax: +81-48-462-4693, E-mail: natori{at}postman.riken.go.jp, URL: http://www.riken.go.jp/engn/r-world/research/lab/special/natori/index.html


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