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J. Biochem, 2004, Vol. 135, No. 2 217-223
© 2004 The Japanese Biochemical Society

MOLECULAR BIOLOGY

Identification of the Transactivating Region of the Homeodomain Protein, Hex

Shinya Kasamatsu1, Ayuko Sato1, Taichi Yamamoto1, Vincent W. Keng1, Hiroshi Yoshida1, Yuka Yamazaki1, Masafumi Shimoda2, Jun-ichi Miyazaki2 and Tamio Noguchi*,1

1 Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601; and 2 Division of Stem Cell Regulation Research, Osaka University Medical School, Suita, Osaka 565-0871

The homeodomain-containing protein Hex acts as an activator as well as a repressor of transcription in animals. While its repression domain has been mapped to the amino-terminal region, the activation domain has never been identified. Here, we show that the homeodomain and the acidic carboxyl-terminal region are necessary for full activation of the sodium-dependent bile acid cotransporter gene promoter in a cell type-independent manner, suggesting that the carboxyl-terminal region comprising residues 197 to 271 functions as the activation domain. In addition, we observed that a Hex mutant without this activation domain acts as a dominant-negative mutant as to the transactivating function of Hex.

* To whom correspondence should be addressed. Fax: +81-52-789-4121, E-mail: tnoguchi{at}agr.nagoya-u.ac.jp


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