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J. Biochem, 2004, Vol. 135, No. 3 331-335
© 2004 The Japanese Biochemical Society

CELL

Overexpression of DRG2 Increases G2/M Phase Cells and Decreases Sensitivity to Nocodazole-Induced Apoptosis

Hebok Song1, Sin-Il Kim1, Myoung Seok Ko2, Hyo Jeong Kim2, Jin Chul Heo1, Hae Jin Lee1, Han Saem Lee2, In Seob Han2, KyuBum Kwack3 and Jeong Woo Park*,2

1 Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea; 2 Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Korea; and 3 Central Genome Center, National Institute of Health, 5 Nokbun-dong, Eunpyung-ku, Seoul 122-701, Korea

DRG2, a member of the DRG subfamily in the GTP-binding protein superfamily, was identified as a repressed gene product in fibroblasts transformed by SV40. The significance of this down-regulation and the cellular role of DRG2 has not been understood in the past. To investigate the function of DRG2 we made a Jurkat cell line, Jurkat-LNCX2-DRG2, stably transfected with pLNCX2-DRG2 to overexpress human DRG2. Cell cycle distribution analysis revealed an increased accumulation of G2/M phase cells in Jurkat-LNCX2-DRG2 cells, indicating a retardation of cell-cycle progression. In addition, an overexpression of DRG2 reduced the sensitivity of Jurkat cells to the mitotic poison nocodazole. Our data suggest that overexpression of DRG2 in Jurkat cells affects genes regulating cell-cycle arrest and apoptosis, and that these molecular changes may be important in the growth or differentiation of cells.

* To whom correspondence should be addressed. Fax: +82-52-259-1694, E-mail: jwpark{at}uou.ulsan.ac.kr


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