Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Ijichi, N.
Right arrow Articles by Iwaki, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ijichi, N.
Right arrow Articles by Iwaki, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 2004, Vol. 135, No. 3 413-420
© 2004 The Japanese Biochemical Society

MOLECULAR BIOLOGY

Distal Sox Binding Elements of the {alpha}B-Crystallin Gene Show Lens Enhancer Activity in Transgenic Mouse Embryos

Nobuhiro Ijichi1, Naomi Tsujimoto1, Toru Iwaki2, Yasuyuki Fukumaki1 and Akiko Iwaki*,1

1 Division of Disease Genes, Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582; and 2 Department of Neuropathology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582

{alpha}B-Crystallin, a member of the small heat shock protein (sHSP) family, is expressed in various tissues including lens, heart, and skeletal muscle. Previously we identified the gene of HSPB2, another member of the sHSP family, located 1-kb upstream of the {alpha}B-crystallin gene in a head-to-head manner. In the present study, we found a highly conserved region of 220 bp approximately 2.4-kb upstream of the {alpha}B-crystallin gene and examined its role in expression of the {alpha}B-crystallin gene. Transgenic mice containing 3 kb of the upstream sequence of the {alpha}B-crystallin gene showed lacZ reporter gene expression in the lens as well as the myotome and heart on embryonic day 12.5. Deletion analysis revealed that the –2656/–2267 region including the conserved region with four putative Sox binding elements (E1–E4) exhibits lens enhancer activity toward the {alpha}B-crystallin promoter. Gel shift assays showed that the Sox1 and Sox2 proteins preferentially bound to E2 and E4. Moreover, disruption of E2 and E4 abolished the reporter gene expression in the lens. These results indicate that the newly identified enhancer with Sox elements activates the {alpha}B-crystallin promoter in the lens, although they are separated by the entire HSPB2 gene.

* To whom correspondence should be addressed. Tel: +81-92-642-6168, Fax: +81-92-632-2375, E-mail: aiwaki{at}gen.kyushu-u.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DNA ResHome page
A. A. Sharov, D. B. Dudekula, and M. S. H. Ko
CisView: A Browser and Database of cis-regulatory Modules Predicted in the Mouse Genome
DNA Res, January 1, 2006; 13(3): 123 - 134.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.