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J. Biochem, 2004, Vol. 135, No. 6 683-693
© 2004 The Japanese Biochemical Society

MOLECULAR BIOLOGY

Bombyx Y-Box Protein BYB Facilitates Specific DNA Interaction of Various DNA Binding Proteins Independently of the Cold Shock Domain

Shigeharu Takiya1,2,*, Yoshinori Nishita1, Susumu Ishikawa2,§, Kaoru Ohno3, Taka-aki Tamura4 and Yoshiaki Suzuki3

1 Laboratory of Gene Function and Regulation, CAST, Hokkaido University, North 10, West 8, and 2 Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, North 10, West 5, Kita-ku, Sapporo 060-0810; 3 Department of Developmental Biology, National Institute for Basic Biology, Myodaiji-cho, Okazaki 444-8585; and 4 Department of Biology, Faculty of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522

A new member of the Y-box protein family of the silkworm Bombyx mori (BYB) was co-purified with the fibroin gene enhancer-binding protein FMBP-1, and stimulated the binding of FMBP-1 to its cognate DNA element. However, the stimulatory effect was not specific to FMBP-1, BYB also enhancing the binding of mammalian transcription factors OTF2, SP1 and AP2 to their specific binding elements. Besides the above transcription regulatory factors, BYB facilitated the binding of basal transcription factor TBP, and enhanced transcription from the adenovirus 2 major late promoter in a reconstituted transcription system. Moreover, BYB stimulated the reactions of some restriction endonucleases under cold conditions. The C-terminal region of BYB was sufficient for these stimulatory effects, and the highly conserved cold shock domain (CSD) in the N-terminal region was dispensable. GST-pull down experiments showed that the C-terminal region could interact with DNA independently of the CSD. The above results suggest that the C-terminal region of BYB causes the active interaction of various DNA binding proteins with their targets. Such a function of the C-terminal region of BYB may partly explain the functional diversity of Y-box proteins.

* To whom correspondence should be addressed. Tel: +81-11-706-2798, Fax: +81-11-737-0536, E-mail: takiya{at}rmg.hokudai.ac.jp

§ Present address: Institute for Genetic Medicine, Hokkaido University, North 15, West 7, Kita-ku, Sapporo 060-0815.


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This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Takiya, T. Ishikawa, K. Ohtsuka, Y. Nishita, and Y. Suzuki
Fibroin-modulator-binding protein-1 (FMBP-1) contains a novel DNA-binding domain, repeats of the score and three amino acid peptide (STP), conserved from Caenorhabditis elegans to humans
Nucleic Acids Res., February 1, 2005; 33(2): 786 - 795.
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