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J. Biochem, 2004, Vol. 136, No. 1 73-79
© 2004 The Japanese Biochemical Society

BIOTECHNOLOGY

Construction and Function of Two Cys146-Mutants with High Activity, Derived from Recombinant Human Soluble B Lymphocyte Stimulator

Guangyu Chen, Shanyun Peng, Minji Zou, Honglian Xu, Donggang Xu and Jiaxi Wang*

Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, China 100850

B lymphocyte stimulator (BLyS) is a novel member of tumor necrosis factor (TNF) ligand family that is important in B cell maturation and survival. Previous studies were almost related to the function or mechanism of its wild type. Here, we constructed two site-directed mutants of the recombinant human soluble BLyS, the BY-A and BY-V, and found that BY-V ranked the highest whenever in the process of promoting proliferation of B lymphocytes in vitro or stimulating total serum IgG and IgM secretion in vivo. Besides, assays for the biological responses of human leukemic cell lines to BLyS, BY-A and BY-V demonstrated that they could suppress the proliferation of Raji cells but promote the growth of THP-1. The discovery of BY-V with high activity will help come to a conclusion that the mutation of Cys146 to Val146 might improve the biological activity of BLyS.

* To whom correspondence should be addressed. Tel: +86-10-66931322, Fax: +86-10-68213039, E-mail: wangjx20000{at}sina.com


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