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J. Biochem, 2004, Vol. 136, No. 2 183-188
© 2004 The Japanese Biochemical Society

MOLECULAR BIOLOGY

Identification and Characterization of Nuclear Localization Signals of CaMKP-N

Masayuki Takeuchi*, Takanobu Taniguchi and Hitoshi Fujisawa§

Department of Biochemistry, Asahikawa Medical College, Asahikawa 078-8510

Calmodulin-dependent protein kinase phosphatase (CaMKP) and CaMKP-N dephosphorylate and regulate multifunctional Ca2+/calmodulin-dependent protein kinases. The enzymatic properties of CaMKP-N and CaMKP resemble each other, whereas their localizations are different. CaMKP-N is localized in the nucleus, whereas CaMKP is localized in the cytosol. In the present study, the nuclear localization signals (NLSs) of CaMKP-N were identified and characterized. CaMKP-N contains two NLSs, NLS1 and NLS2, at the C-terminus. A cluster of basic residues in the NLSs is important for their function. NLS1 and NLS2 function independently, but mutagenesis analysis suggests that these NLSs interact with each other.

* To whom correspondence should be addressed. Tel: +81-166-68-2342, Fax: +81-166-68-2349, E-mail: mtake{at}asahikawa-med.ac.jp

§ Present address: Shiga Medical Center Research Institute, 4-30 Moriyama 5-chome, Moriyama, Shiga 524-8524, Japan.


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