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J. Biochem, 2004, Vol. 136, No. 2 239-243
© 2004 The Japanese Biochemical Society

BIOCHEMISTRY

Isoosmotic Isolation of Rat Brain Synaptic Vesicles, Some of Which Contain Tyrosine Hydroxylase

Toshihiro Tsudzuki* and Maki Tsujita

Department of Biochemistry I, Nagoya City University Medical School, Kawasumi 1, Mizuho-ku, Nagoya 467-8601

Rat brain synaptic vesicles were isoosmotically isolated and examined for Mg2+-ATPase [EC 3.6.1.3.] and tyrosine hydroxylase [EC 1.14.16.2.] associated with the synaptic vesicles. Synaptosomes in 0.32 M sucrose were disrupted by freezing and thawing treatment, and the cytosol fraction was fractionated on a Sephacryl S-500 column with a mean exclusion size of 200 nm. Peak I at the void volume was a mixture of large vesicular membranes, small amounts of synaptic vesicles and coated vesicles, etc. Peak II consisted of non- and granulated synaptic vesicles of 35–40 nm diameter, and peak III of soluble proteins. The synaptic vesicles in peak II reacted with antibodies against the H+-ATPase A-subunit, vesicular acetylcholine transporter, and vesicular monoamine transporter. However, they showed little Mg2+-ATPase activity. Tyrosine hydroxylase was observed in either peak II or III on blotting with an anti-tyrosine hydroxylase antibody. These results imply that tyrosine hydroxylase exists in soluble and bound forms to synaptic vesicles in nerve terminals.

* To whom correspondence should be addressed. Tel: +81-52-853-8141, Fax: +81-52-841-3480


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