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J. Biochem, 2004, Vol. 136, No. 2 255-259
© 2004 The Japanese Biochemical Society

BIOCHEMISTRY

Induction of Transient Ion Channel-Like Pores in a Cancer Cell by Antibiotic Peptide

Jian-Shan Ye1, Xiao-Jing Zheng2, King Wong Leung2, Hueih Min Chen3,* and Fwu-Shan Sheu1,4,*

1 Department of Biological Sciences, 14 Science Drive 4, National University of Singapore, Singapore 117543, Singapore; 2 Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PR China; 3 Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan; and 4 The University Scholars Program, 10 Kent Ridge Crescent, National University of Singapore, Singapore 119260, Singapore

The anticancer activity of anti-bacterial cecropins makes them potentially useful as peptide anti-cancer drugs. We used the cell-attached patch to study the effect of cecropin B (CB; having one hydrophobic and one amphipathic {alpha}-helix) and its derivative, cecropin B3 (CB3; having two hydrophobic {alpha}-helices) on the membrane of Ags cancer cells. Application of 10–60 µM CB onto the membrane of the cancer cell produces short outward currents. Comparative study with CB3, which induces no outward currents, shows that the amphipathic group of CB is necessary for the pore formation. The results provide a rationale to study the cell-killing activity of antimicrobial peptides at the single cancer cell level.

* To whom correspondence should be addressed. F.-S.S.: Fax: +65-67792486, E-mail: dbssfs{at}nus.edu.sg; H.M.C.: Fax: +886-2-2789-8629, E-mail: robell{at}gate.sinica.edu.tw


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